Review



anti fancd2  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Novus Biologicals anti fancd2
    Anti Fancd2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody+(FI-17)/us12558369-285-24-25
    Average 94 stars, based on 32 article reviews
    anti fancd2 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    other:

    Article Title: Topoisomerase 2β Induces DNA Breaks To Regulate Human Papillomavirus Replication
    Article Snippet: Antibodies to phosphorylated CHK1 (pCHK1) (catalog no. 12302; Cell Signaling Technology [CST]), phosphorylated CHK2 (pCHK2) (catalog no. 2661; CST), FANCD2 (catalog no. 100182; Novus), phosphorylated H2Ax (gH2AX) (catalog no. 05636; Millipore), phosphorylated SMC1 (pSMC1) (catalog no. 4805S; CST), BRCA1 (catalog no. OP92; Millipore), RAD51 (catalog no. NB100148; Novus), and TOP2b (catalog no. A300-950A; Bethyl Laboratories) were used.

    Article Title: TFG-maintaining stability of overlooked FANCD2 confers early DNA-damage response
    Article Snippet: Those recognizing FANCD2, TFG or RFP were obtained from NOVUS (Littleton, CO).

    Article Title: Topoisomerase 2β Induces DNA Breaks To Regulate Human Papillomavirus Replication
    Article Snippet: Antibodies to phosphorylated CHK1 (pCHK1) (catalog no. 12302; Cell Signaling Technology [CST]), phosphorylated CHK2 (pCHK2) (catalog no. 2661; CST), FANCD2 (catalog no. 100182; Novus), phosphorylated H2Ax (γH2AX) (catalog no. 05636; Millipore), phosphorylated SMC1 (pSMC1) (catalog no. 4805S; CST), BRCA1 (catalog no. OP92; Millipore), RAD51 (catalog no. NB100148; Novus), and TOP2β (catalog no.A300-950A; Bethyl Laboratories) were used.

    Incubation:

    Article Title: Deficiency of the Fanconi anemia core complex protein FAAP100 results in severe Fanconi anemia
    Article Snippet: Immunoblotting was performed using the following antibodies: FANCD2 (Novus, NB100-182, lot 8-9); HA (BioLegend, clone 16B12, lot B379455); and FAAP100 (gift from Weidong Wang, NIH, Laboratory of Genetics and Genomics, National Institute on Aging). .. Cells were fixed in 3.7% formaldehyde and permeabilized with 0.5% Triton in PBS, blocked in 5% (v/v) FBS in PBS, and incubated with FANCD2 antibody (Novus, NB100-182, lot 8-9) at 1:2000 dilution in blocking buffer. .. Cells were washed and incubated with Alexa-Fluor 488 secondary antibody (Thermo Fisher Scientific, A11008, lot 645151).

    Article Title: A distinct role for recombination repair factors in an early cellular response to transcription–replication conflicts
    Article Snippet: .. Cells were blocked in PBS-T (PBS with 0.1% Tween20) with 3% BSA (A0281, Sigma-Aldrich) for 1 h before overnight incubation with primary FANCD2 antibody (NB100-182SS, Novus Biologicals) at 4°C. .. Unbound primary antibodies were removed by washing three times for 5 min in PBS followed by incubation with Alexa 568 goat anti-rabbit IgG secondary antibody (1:500, A11011, Life Technologies) for 50 min. Coverslips were then washed three times for 5 min in PBS before mounting with mounting medium containing DAPI (4% n-propyl gallate, 80% glycerol, 1 μg/ml DAPI).

    Blocking Assay:

    Article Title: Deficiency of the Fanconi anemia core complex protein FAAP100 results in severe Fanconi anemia
    Article Snippet: Immunoblotting was performed using the following antibodies: FANCD2 (Novus, NB100-182, lot 8-9); HA (BioLegend, clone 16B12, lot B379455); and FAAP100 (gift from Weidong Wang, NIH, Laboratory of Genetics and Genomics, National Institute on Aging). .. Cells were fixed in 3.7% formaldehyde and permeabilized with 0.5% Triton in PBS, blocked in 5% (v/v) FBS in PBS, and incubated with FANCD2 antibody (Novus, NB100-182, lot 8-9) at 1:2000 dilution in blocking buffer. .. Cells were washed and incubated with Alexa-Fluor 488 secondary antibody (Thermo Fisher Scientific, A11008, lot 645151).

    Lysis:

    Article Title: FANCD2 modulates the mitochondrial stress response to prevent common fragile site instability
    Article Snippet: ChIP-seq experiments were performed using Active Motif ChIP sequencing services. .. First, 1 × 10 7 HCT116 cells that had been treated with or without 0.3 μM APH were fixed in 1% formaldehyde for 15 min. After cell lysis, 30 μg of chromatin was used for immunoprecipitation using a FANCD2 antibody (Novus). .. Immunoprecipitated and input DNA were sequenced by Illumina sequencing, which generated 75-nt sequence reads.

    Immunoprecipitation:

    Article Title: FANCD2 modulates the mitochondrial stress response to prevent common fragile site instability
    Article Snippet: ChIP-seq experiments were performed using Active Motif ChIP sequencing services. .. First, 1 × 10 7 HCT116 cells that had been treated with or without 0.3 μM APH were fixed in 1% formaldehyde for 15 min. After cell lysis, 30 μg of chromatin was used for immunoprecipitation using a FANCD2 antibody (Novus). .. Immunoprecipitated and input DNA were sequenced by Illumina sequencing, which generated 75-nt sequence reads.

    Article Title: FANCD2 modulates the mitochondrial stress response to prevent common fragile site instability
    Article Snippet: .. After preclearing with magnetic beads for 1 h, the chromatin from an equivalent of 1 × 10 7 HCT116 cells was used for immunoprecipitation with a FANCD2 antibody (Novus) or immunoglobulin G as a control. .. After an overnight incubation at 4 °C, the beads were washed and eluted in buffer E (25 mM Tris–HCl [pH 7.5], 5 mM EDTA, and 0.5% SDS), and crosslinking was reversed at 65 °C with proteinase K for 6 h. The DNA was then purified using a QIAquick PCR purification kit (QIAGEN) and eluted in 100 μl of distilled water.

    Magnetic Beads:

    Article Title: FANCD2 modulates the mitochondrial stress response to prevent common fragile site instability
    Article Snippet: .. After preclearing with magnetic beads for 1 h, the chromatin from an equivalent of 1 × 10 7 HCT116 cells was used for immunoprecipitation with a FANCD2 antibody (Novus) or immunoglobulin G as a control. .. After an overnight incubation at 4 °C, the beads were washed and eluted in buffer E (25 mM Tris–HCl [pH 7.5], 5 mM EDTA, and 0.5% SDS), and crosslinking was reversed at 65 °C with proteinase K for 6 h. The DNA was then purified using a QIAquick PCR purification kit (QIAGEN) and eluted in 100 μl of distilled water.

    Control:

    Article Title: FANCD2 modulates the mitochondrial stress response to prevent common fragile site instability
    Article Snippet: .. After preclearing with magnetic beads for 1 h, the chromatin from an equivalent of 1 × 10 7 HCT116 cells was used for immunoprecipitation with a FANCD2 antibody (Novus) or immunoglobulin G as a control. .. After an overnight incubation at 4 °C, the beads were washed and eluted in buffer E (25 mM Tris–HCl [pH 7.5], 5 mM EDTA, and 0.5% SDS), and crosslinking was reversed at 65 °C with proteinase K for 6 h. The DNA was then purified using a QIAquick PCR purification kit (QIAGEN) and eluted in 100 μl of distilled water.



    Similar Products

    93
    Proteintech fancd2
    Fancd2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody/pmc12936848-59-0-2
    Average 93 stars, based on 1 article reviews
    fancd2 - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    94
    Santa Cruz Biotechnology rad51
    Inhibition of NAMPT function via KPT-9274 inhibits tumor growth in vivo. (A) NOG mice were injected subcutaneously with Mino cells. After detection of the tumor, the mice were randomized and treated orally with either KPT-9274 or the vehicle for 5 consecutive days per week for 3 weeks. Tumor volume was evaluated via caliper measurement. Differences between the 2 groups were evaluated using the standard t test. (B) Tumor cells collected from mice were lysed in radio-immunoprecipitation assay buffer, and the whole-cell lysate was subjected to WB analysis and probed with antibodies against Cl-PARP, FANCD2, <t>RAD51,</t> and γ-H2AX. GAPDH was used as the loading control. ns > .05; ∗∗ P ≤ .01; ∗∗∗ P ≤ .001; ∗∗∗∗ P ≤ .0001.
    Rad51, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody/pmc12964014-41-25-26
    Average 94 stars, based on 1 article reviews
    rad51 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Santa Cruz Biotechnology fancd2
    Inhibition of NAMPT function via KPT-9274 inhibits tumor growth in vivo. (A) NOG mice were injected subcutaneously with Mino cells. After detection of the tumor, the mice were randomized and treated orally with either KPT-9274 or the vehicle for 5 consecutive days per week for 3 weeks. Tumor volume was evaluated via caliper measurement. Differences between the 2 groups were evaluated using the standard t test. (B) Tumor cells collected from mice were lysed in radio-immunoprecipitation assay buffer, and the whole-cell lysate was subjected to WB analysis and probed with antibodies against Cl-PARP, <t>FANCD2,</t> RAD51, and γ-H2AX. GAPDH was used as the loading control. ns > .05; ∗∗ P ≤ .01; ∗∗∗ P ≤ .001; ∗∗∗∗ P ≤ .0001.
    Fancd2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody/pmc12964014-41-20-21
    Average 94 stars, based on 1 article reviews
    fancd2 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    94
    Novus Biologicals anti fancd2
    Inhibition of NAMPT function via KPT-9274 inhibits tumor growth in vivo. (A) NOG mice were injected subcutaneously with Mino cells. After detection of the tumor, the mice were randomized and treated orally with either KPT-9274 or the vehicle for 5 consecutive days per week for 3 weeks. Tumor volume was evaluated via caliper measurement. Differences between the 2 groups were evaluated using the standard t test. (B) Tumor cells collected from mice were lysed in radio-immunoprecipitation assay buffer, and the whole-cell lysate was subjected to WB analysis and probed with antibodies against Cl-PARP, <t>FANCD2,</t> RAD51, and γ-H2AX. GAPDH was used as the loading control. ns > .05; ∗∗ P ≤ .01; ∗∗∗ P ≤ .001; ∗∗∗∗ P ≤ .0001.
    Anti Fancd2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody+(FI-17)/us12558369-285-24-25
    Average 94 stars, based on 1 article reviews
    anti fancd2 - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    95
    Novus Biologicals fancd2
    Replication stress and DNA damage response at CTCF/cohesin-binding sites in normal cells (A–D) ChIP-seq signal profile and heatmap of (A) MRE11, (B) STN1+HU 3h, (C) γH2AX (normalized with H2AX), and (D) RAD51 in the Mid S phase of HeLa cells plotted at CBSs shared between HeLa and normal cells (H1, IMR90, and RPE1 cells) (12,517 sites) and HeLa-specific CBSs (2,293 sites) and normal-specific sites (13,702). For the γH2AX signal ±10 kb flanks were considered, while for others the signal is plotted at ±5 kb regions. (E–H) ChIP-qPCR plots of (E) MRE11, (F) <t>FANCD2,</t> (G) γH2AX, and (H) RAD51 in Mid S synchronized hTERT RPE-1 cells at CBSs and CTCF-unbound sites. The y axis (fold enrichment over beads) indicates the % input in immunoprecipitation divided by that of beads. The bar represents the mean value from three replicates, and the error bar represents the standard error of the mean. Statistical significance was determined by using a two-sided Mann-Whitney U test.
    Fancd2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody+-+BSA+Free/pmc12860730-403-41-43
    Average 95 stars, based on 1 article reviews
    fancd2 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Novus Biologicals nb 100 182 rrid ab 350110
    Replication stress and DNA damage response at CTCF/cohesin-binding sites in normal cells (A–D) ChIP-seq signal profile and heatmap of (A) MRE11, (B) STN1+HU 3h, (C) γH2AX (normalized with H2AX), and (D) RAD51 in the Mid S phase of HeLa cells plotted at CBSs shared between HeLa and normal cells (H1, IMR90, and RPE1 cells) (12,517 sites) and HeLa-specific CBSs (2,293 sites) and normal-specific sites (13,702). For the γH2AX signal ±10 kb flanks were considered, while for others the signal is plotted at ±5 kb regions. (E–H) ChIP-qPCR plots of (E) MRE11, (F) <t>FANCD2,</t> (G) γH2AX, and (H) RAD51 in Mid S synchronized hTERT RPE-1 cells at CBSs and CTCF-unbound sites. The y axis (fold enrichment over beads) indicates the % input in immunoprecipitation divided by that of beads. The bar represents the mean value from three replicates, and the error bar represents the standard error of the mean. Statistical significance was determined by using a two-sided Mann-Whitney U test.
    Nb 100 182 Rrid Ab 350110, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody+-+BSA+Free/pmc12860730-10-7-4
    Average 95 stars, based on 1 article reviews
    nb 100 182 rrid ab 350110 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Novus Biologicals rabbit anti fancd2 antibody
    (A) Western blot illustrating FANCA protein loss in FANCA-deficient cell lines. (B,C) Lack of <t>FANCD2</t> monoubiquitination, a hallmark of FA pathway activation, in FANCA-deficient cells. (D,E) FANCA-deficient cells show increased vulnerability to MMC: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (D) and DU145 WT vs DU145_ FANCA KO (E) are illustrated. (F,G) FANCA-deficient cells show increased vulnerability to cisplatin: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (F) and DU145 WT vs DU145_ FANCA KO (G) are illustrated.
    Rabbit Anti Fancd2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody+-+BSA+Free/bio_rxiv__64898__2026__02__04__703705-167-13-16
    Average 95 stars, based on 1 article reviews
    rabbit anti fancd2 antibody - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    94
    Novus Biologicals mouse anti fancd2 antibody
    (A) Western blot illustrating FANCA protein loss in FANCA-deficient cell lines. (B,C) Lack of <t>FANCD2</t> monoubiquitination, a hallmark of FA pathway activation, in FANCA-deficient cells. (D,E) FANCA-deficient cells show increased vulnerability to MMC: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (D) and DU145 WT vs DU145_ FANCA KO (E) are illustrated. (F,G) FANCA-deficient cells show increased vulnerability to cisplatin: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (F) and DU145 WT vs DU145_ FANCA KO (G) are illustrated.
    Mouse Anti Fancd2 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fancd2+antibody/FANCD2+Antibody+(FI-17)/bio_rxiv__64898__2026__02__04__703705-167-23-26
    Average 94 stars, based on 1 article reviews
    mouse anti fancd2 antibody - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of NAMPT function via KPT-9274 inhibits tumor growth in vivo. (A) NOG mice were injected subcutaneously with Mino cells. After detection of the tumor, the mice were randomized and treated orally with either KPT-9274 or the vehicle for 5 consecutive days per week for 3 weeks. Tumor volume was evaluated via caliper measurement. Differences between the 2 groups were evaluated using the standard t test. (B) Tumor cells collected from mice were lysed in radio-immunoprecipitation assay buffer, and the whole-cell lysate was subjected to WB analysis and probed with antibodies against Cl-PARP, FANCD2, RAD51, and γ-H2AX. GAPDH was used as the loading control. ns > .05; ∗∗ P ≤ .01; ∗∗∗ P ≤ .001; ∗∗∗∗ P ≤ .0001.

    Journal: Blood Advances

    Article Title: Inhibition of NAMPT targets DNA damage response to sensitize alkylating chemotherapy in TP53 mutant mantle cell lymphoma ∗

    doi: 10.1182/bloodadvances.2025016765

    Figure Lengend Snippet: Inhibition of NAMPT function via KPT-9274 inhibits tumor growth in vivo. (A) NOG mice were injected subcutaneously with Mino cells. After detection of the tumor, the mice were randomized and treated orally with either KPT-9274 or the vehicle for 5 consecutive days per week for 3 weeks. Tumor volume was evaluated via caliper measurement. Differences between the 2 groups were evaluated using the standard t test. (B) Tumor cells collected from mice were lysed in radio-immunoprecipitation assay buffer, and the whole-cell lysate was subjected to WB analysis and probed with antibodies against Cl-PARP, FANCD2, RAD51, and γ-H2AX. GAPDH was used as the loading control. ns > .05; ∗∗ P ≤ .01; ∗∗∗ P ≤ .001; ∗∗∗∗ P ≤ .0001.

    Article Snippet: Western blotting (WB) was performed to evaluate the expression levels of total protein and phospho-specific isoforms using the following antibodies: FANCD2 (Santa Cruz Biotechnology, sc-20022), RAD51 (Santa Cruz Biotechnology, sc-398587), PBEF (Santa Cruz Biotechnology, sc-166946), cleaved Caspase3 (Cell Signaling Technology, 9664s), PARP (Cell Signaling Technology, 9532s), cleaved PARP (Cell Signaling Technology, 5625s), γ-H2AX (Ser139; Cell Signaling Technology, 9718s), p-CHK1 (Ser345; Cell Signaling Technology, 2341s), p53 (Cell Signaling Technology, 2527s), p-ATR (Ser428; Cell Signaling Technology, 2853s), p-ATM (Ser1981; Cell Signaling Technology, 4526s), and p-CHK2 (Thr68; Cell Signaling Technology, 2197s).

    Techniques: Inhibition, In Vivo, Injection, Radio Immunoprecipitation, Control

    Inhibition of NAMPT function via KPT-9274 inhibits tumor growth in vivo. (A) NOG mice were injected subcutaneously with Mino cells. After detection of the tumor, the mice were randomized and treated orally with either KPT-9274 or the vehicle for 5 consecutive days per week for 3 weeks. Tumor volume was evaluated via caliper measurement. Differences between the 2 groups were evaluated using the standard t test. (B) Tumor cells collected from mice were lysed in radio-immunoprecipitation assay buffer, and the whole-cell lysate was subjected to WB analysis and probed with antibodies against Cl-PARP, FANCD2, RAD51, and γ-H2AX. GAPDH was used as the loading control. ns > .05; ∗∗ P ≤ .01; ∗∗∗ P ≤ .001; ∗∗∗∗ P ≤ .0001.

    Journal: Blood Advances

    Article Title: Inhibition of NAMPT targets DNA damage response to sensitize alkylating chemotherapy in TP53 mutant mantle cell lymphoma ∗

    doi: 10.1182/bloodadvances.2025016765

    Figure Lengend Snippet: Inhibition of NAMPT function via KPT-9274 inhibits tumor growth in vivo. (A) NOG mice were injected subcutaneously with Mino cells. After detection of the tumor, the mice were randomized and treated orally with either KPT-9274 or the vehicle for 5 consecutive days per week for 3 weeks. Tumor volume was evaluated via caliper measurement. Differences between the 2 groups were evaluated using the standard t test. (B) Tumor cells collected from mice were lysed in radio-immunoprecipitation assay buffer, and the whole-cell lysate was subjected to WB analysis and probed with antibodies against Cl-PARP, FANCD2, RAD51, and γ-H2AX. GAPDH was used as the loading control. ns > .05; ∗∗ P ≤ .01; ∗∗∗ P ≤ .001; ∗∗∗∗ P ≤ .0001.

    Article Snippet: Western blotting (WB) was performed to evaluate the expression levels of total protein and phospho-specific isoforms using the following antibodies: FANCD2 (Santa Cruz Biotechnology, sc-20022), RAD51 (Santa Cruz Biotechnology, sc-398587), PBEF (Santa Cruz Biotechnology, sc-166946), cleaved Caspase3 (Cell Signaling Technology, 9664s), PARP (Cell Signaling Technology, 9532s), cleaved PARP (Cell Signaling Technology, 5625s), γ-H2AX (Ser139; Cell Signaling Technology, 9718s), p-CHK1 (Ser345; Cell Signaling Technology, 2341s), p53 (Cell Signaling Technology, 2527s), p-ATR (Ser428; Cell Signaling Technology, 2853s), p-ATM (Ser1981; Cell Signaling Technology, 4526s), and p-CHK2 (Thr68; Cell Signaling Technology, 2197s).

    Techniques: Inhibition, In Vivo, Injection, Radio Immunoprecipitation, Control

    Replication stress and DNA damage response at CTCF/cohesin-binding sites in normal cells (A–D) ChIP-seq signal profile and heatmap of (A) MRE11, (B) STN1+HU 3h, (C) γH2AX (normalized with H2AX), and (D) RAD51 in the Mid S phase of HeLa cells plotted at CBSs shared between HeLa and normal cells (H1, IMR90, and RPE1 cells) (12,517 sites) and HeLa-specific CBSs (2,293 sites) and normal-specific sites (13,702). For the γH2AX signal ±10 kb flanks were considered, while for others the signal is plotted at ±5 kb regions. (E–H) ChIP-qPCR plots of (E) MRE11, (F) FANCD2, (G) γH2AX, and (H) RAD51 in Mid S synchronized hTERT RPE-1 cells at CBSs and CTCF-unbound sites. The y axis (fold enrichment over beads) indicates the % input in immunoprecipitation divided by that of beads. The bar represents the mean value from three replicates, and the error bar represents the standard error of the mean. Statistical significance was determined by using a two-sided Mann-Whitney U test.

    Journal: iScience

    Article Title: CTCF/cohesin-binding sites are susceptible to replication-associated DNA damage and genomic instability in cancer cells

    doi: 10.1016/j.isci.2026.114646

    Figure Lengend Snippet: Replication stress and DNA damage response at CTCF/cohesin-binding sites in normal cells (A–D) ChIP-seq signal profile and heatmap of (A) MRE11, (B) STN1+HU 3h, (C) γH2AX (normalized with H2AX), and (D) RAD51 in the Mid S phase of HeLa cells plotted at CBSs shared between HeLa and normal cells (H1, IMR90, and RPE1 cells) (12,517 sites) and HeLa-specific CBSs (2,293 sites) and normal-specific sites (13,702). For the γH2AX signal ±10 kb flanks were considered, while for others the signal is plotted at ±5 kb regions. (E–H) ChIP-qPCR plots of (E) MRE11, (F) FANCD2, (G) γH2AX, and (H) RAD51 in Mid S synchronized hTERT RPE-1 cells at CBSs and CTCF-unbound sites. The y axis (fold enrichment over beads) indicates the % input in immunoprecipitation divided by that of beads. The bar represents the mean value from three replicates, and the error bar represents the standard error of the mean. Statistical significance was determined by using a two-sided Mann-Whitney U test.

    Article Snippet: Antibodies used are: CTCF (3418: Cell Signaling Technology, 1 μg), RAD21 (ab992: Abcam, 1 μg), MRE11 (ab208020: Abcam, 2 μg), γH2AX (ab81299: Abcam, 2 μg), H2AX (ab11175: Abcam, 2 μg), RAD51 (ab176458: Abcam, 2 μg), ATM (ab201022: Abcam, 2 μg) and FANCD2 (NB100-182: Novus Biologicals, 2 μg).

    Techniques: Binding Assay, ChIP-sequencing, ChIP-qPCR, Immunoprecipitation, MANN-WHITNEY

    (A) Western blot illustrating FANCA protein loss in FANCA-deficient cell lines. (B,C) Lack of FANCD2 monoubiquitination, a hallmark of FA pathway activation, in FANCA-deficient cells. (D,E) FANCA-deficient cells show increased vulnerability to MMC: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (D) and DU145 WT vs DU145_ FANCA KO (E) are illustrated. (F,G) FANCA-deficient cells show increased vulnerability to cisplatin: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (F) and DU145 WT vs DU145_ FANCA KO (G) are illustrated.

    Journal: bioRxiv

    Article Title: Aurora kinase A is a synthetic lethal target in FANCA-deficient cancers

    doi: 10.64898/2026.02.04.703705

    Figure Lengend Snippet: (A) Western blot illustrating FANCA protein loss in FANCA-deficient cell lines. (B,C) Lack of FANCD2 monoubiquitination, a hallmark of FA pathway activation, in FANCA-deficient cells. (D,E) FANCA-deficient cells show increased vulnerability to MMC: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (D) and DU145 WT vs DU145_ FANCA KO (E) are illustrated. (F,G) FANCA-deficient cells show increased vulnerability to cisplatin: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (F) and DU145 WT vs DU145_ FANCA KO (G) are illustrated.

    Article Snippet: Cells were then incubated with the primary antibody for 1 hour at RT, rabbit anti-FANCD2 antibody (Novus Biologicals, NB100-182, 1:500 in SB) or mouse anti-FANCD2 antibody (Novus Biologicals, NB100-316, 1:500 in SB).

    Techniques: Western Blot, Activation Assay

    Cells were exposed to 1 µM MMC for 24 hours vs control conditions (DMSO) and imaged via confocal microscopy using Zeiss LSM 710 or Zeiss LSM 980 (63x). (A,C,E) Representative immunofluorescence images for CCH-SCC-FA1 ( FANCA Compl ) vs CCH-SCC-FA1 ( FANCA -/- ) (A), DU145 WT vs DU145_ FANCA KO (C), and RPE1 WT vs RPE1_ FANCA KD (E); scale represents 20 µm. (B, D, F) FANCD2 foci quantification for the same FANCA-deficient vs proficient cell lines. The number of foci per cell are represented, and statistical significance was calculated with the unpaired t-test.

    Journal: bioRxiv

    Article Title: Aurora kinase A is a synthetic lethal target in FANCA-deficient cancers

    doi: 10.64898/2026.02.04.703705

    Figure Lengend Snippet: Cells were exposed to 1 µM MMC for 24 hours vs control conditions (DMSO) and imaged via confocal microscopy using Zeiss LSM 710 or Zeiss LSM 980 (63x). (A,C,E) Representative immunofluorescence images for CCH-SCC-FA1 ( FANCA Compl ) vs CCH-SCC-FA1 ( FANCA -/- ) (A), DU145 WT vs DU145_ FANCA KO (C), and RPE1 WT vs RPE1_ FANCA KD (E); scale represents 20 µm. (B, D, F) FANCD2 foci quantification for the same FANCA-deficient vs proficient cell lines. The number of foci per cell are represented, and statistical significance was calculated with the unpaired t-test.

    Article Snippet: Cells were then incubated with the primary antibody for 1 hour at RT, rabbit anti-FANCD2 antibody (Novus Biologicals, NB100-182, 1:500 in SB) or mouse anti-FANCD2 antibody (Novus Biologicals, NB100-316, 1:500 in SB).

    Techniques: Control, Confocal Microscopy, Immunofluorescence

    (A) Western blot illustrating FANCA protein loss in FANCA-deficient cell lines. (B,C) Lack of FANCD2 monoubiquitination, a hallmark of FA pathway activation, in FANCA-deficient cells. (D,E) FANCA-deficient cells show increased vulnerability to MMC: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (D) and DU145 WT vs DU145_ FANCA KO (E) are illustrated. (F,G) FANCA-deficient cells show increased vulnerability to cisplatin: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (F) and DU145 WT vs DU145_ FANCA KO (G) are illustrated.

    Journal: bioRxiv

    Article Title: Aurora kinase A is a synthetic lethal target in FANCA-deficient cancers

    doi: 10.64898/2026.02.04.703705

    Figure Lengend Snippet: (A) Western blot illustrating FANCA protein loss in FANCA-deficient cell lines. (B,C) Lack of FANCD2 monoubiquitination, a hallmark of FA pathway activation, in FANCA-deficient cells. (D,E) FANCA-deficient cells show increased vulnerability to MMC: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (D) and DU145 WT vs DU145_ FANCA KO (E) are illustrated. (F,G) FANCA-deficient cells show increased vulnerability to cisplatin: drug response curves for CCH-SCC-FA1 ( FANCA -/- ) vs CCH-SCC-FA1 ( FANCA Compl ) (F) and DU145 WT vs DU145_ FANCA KO (G) are illustrated.

    Article Snippet: Cells were then incubated with the primary antibody for 1 hour at RT, rabbit anti-FANCD2 antibody (Novus Biologicals, NB100-182, 1:500 in SB) or mouse anti-FANCD2 antibody (Novus Biologicals, NB100-316, 1:500 in SB).

    Techniques: Western Blot, Activation Assay

    Cells were exposed to 1 µM MMC for 24 hours vs control conditions (DMSO) and imaged via confocal microscopy using Zeiss LSM 710 or Zeiss LSM 980 (63x). (A,C,E) Representative immunofluorescence images for CCH-SCC-FA1 ( FANCA Compl ) vs CCH-SCC-FA1 ( FANCA -/- ) (A), DU145 WT vs DU145_ FANCA KO (C), and RPE1 WT vs RPE1_ FANCA KD (E); scale represents 20 µm. (B, D, F) FANCD2 foci quantification for the same FANCA-deficient vs proficient cell lines. The number of foci per cell are represented, and statistical significance was calculated with the unpaired t-test.

    Journal: bioRxiv

    Article Title: Aurora kinase A is a synthetic lethal target in FANCA-deficient cancers

    doi: 10.64898/2026.02.04.703705

    Figure Lengend Snippet: Cells were exposed to 1 µM MMC for 24 hours vs control conditions (DMSO) and imaged via confocal microscopy using Zeiss LSM 710 or Zeiss LSM 980 (63x). (A,C,E) Representative immunofluorescence images for CCH-SCC-FA1 ( FANCA Compl ) vs CCH-SCC-FA1 ( FANCA -/- ) (A), DU145 WT vs DU145_ FANCA KO (C), and RPE1 WT vs RPE1_ FANCA KD (E); scale represents 20 µm. (B, D, F) FANCD2 foci quantification for the same FANCA-deficient vs proficient cell lines. The number of foci per cell are represented, and statistical significance was calculated with the unpaired t-test.

    Article Snippet: Cells were then incubated with the primary antibody for 1 hour at RT, rabbit anti-FANCD2 antibody (Novus Biologicals, NB100-182, 1:500 in SB) or mouse anti-FANCD2 antibody (Novus Biologicals, NB100-316, 1:500 in SB).

    Techniques: Control, Confocal Microscopy, Immunofluorescence